ABSTRACT
Objective To evaluate the influence of hematocrit(HCT),vitamin C(Vc)and galactose on three portable blood glucose meters to provide some reference for clinical selection of appropriate blood glucose me-ter.Methods 20 heparin anticoagulant venous blood samples in the clinical laboratory department of Nanfang Hospital were selected for verifying the accuracy of blood glucose meter.2 healthy volunteers were selected for collecting 5 w hole blood samples in interferent test.Referring to the detection results of Roche automatic bio-chemical analyzer,the Nova StatStrip Xpress glucometer,Bayer Contour Plus glucometer and Roche Accu-chek Performa blood glucose meter were performed the accuracy verification.The influence of HCT,Vc and galac-tose on the detection results of above three portable blood glucose meters were evaluated.Results The detec-tion accuracy of Nova StatStrip Xpress and the Roche Accu-Chek glucometers all conformed to the require-ments of ISO15197:2013 standards.The detection accuracy of Bayer Contour Plus glucometer only conformed to the requirements of ISO15197:2003 standards.Under the interference of different levels of HCT,Vc and galactose,the detection results of Nova StatStrip Xpress glucometer conformed to the requirements of ISO15197:2013 standards;w hen detecting low concentration of blood glucose,the Bayer Contour Plus glucom-eter was interfered by 10 mg/dL Vc,and other detection results conformed to the requirements of ISO15197:2003 standards;the anti-interference performance of Roche Accu-chek Performa glucometer conformed to the
ABSTRACT
Objective To investigate the effect of simulated microgravity on the proliferation and differentiation of the human megakaryocyte cells in vitro. Methods The fourth generation rotating cell culture system (RCCS-4) was used to generate the simulated microgravity environment. The cell viability was assessed by trypan blue staining method. The proliferation of cells was assessed by cell counting method and CCK8 method. The CD41+/CD61+ cells rate and the cells cycle were detected by flow cytometry. The expression levels of thrombopoietin receptor (c-mpl) and transcription factors were detected with RT-PCR. Results After 24, 48, 72 h, culture under simulated microgravity resulted in a significant decrease in the cell number , proliferative activity, cells in the G2/M phase and levels of c-mpl mRNA expression in comparison with that under the normal gravity (P < 0.05). After 48 h and 72 h culture, CD41+/CD61+ cells ratio decreased and RUNX-1 mRNA expression was down-regulated in cells of the group SMG compared with that of the group NG (P < 0.05). Conclusion Microgravity can inhibit the proliferation and differentiation of human megakaryocyte cells in vitro. The mechanism may be that TPO/c-mpl pathway was inhibited by down regulating the expression of c-mpl which transcriptional inhibition lead to.